Postdoctoral Research Scientist: In vivo functioning of pituitary-scale cell networks

11 Feb 2008


The Institut de Genomque Fonctionelle in Montpellier, France, are seeking a highly motivated post-doctoral scientist to investigate the functioning of pituitary-scale endocrine cell networks, following on from our recent demonstration that growth hormone (GH)-secreting cells form a geometrically connected homotypic cell network with robust cell-cell contacts and an architecture closely associated with body growth (Bonnefont et al., 2005 PNAS 102(46):16880-5 ; Med Sci 2006 May;22(5):455-6). The objective of this project will be to examine the organizational functioning of prolactin (PRL)-secreting cells, which form another 3-D network intermingled with the GH cell network. Using a newly-developed cellular in vivo approach applied on anesthetized transgenic PRL-DsRed mice, we will assess how the geometry of the PRL cell network influences PRL cell relationships with blood vessels and extracellular matrix, monitor electrophysiological and cytosolic calcium activities within the network of cells and correlate these with PRL secretion in response to physiological demands (oestrus cycle, pregnancy and lactation). Key skills will include cellular fluorescence imaging, two-photon excitation microscopy, genotyping and immunohistochemistry. Experience in electrophysiology will be a plus. The successful applicant will join an interactive, multidisciplinary team in the Department of Endocrinology of the Institute of Functional Genomics. This 2-year position is funded by the “Agence Nationale pour le Recherche” (ANR) and the project is part of a multinational consortium and imaging platforms . The successful applicant will be actively involved in collaborative work with other participating laboratories, which also provide opportunities to exploit state-of-art imaging, genomics and mouse transgenic techniques. Application should include a letter of motivation, CV, summary of current and past research experience, and two professional references. The salary will be determined following ANR guidelines. The position will be available starting from 1 April 2008. Contact: Patrice MOLLARD; email: [email protected]; Phone: +33(0)467142925

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